Table 3 Yeast strains and plasmids used in this study
Strains | Genotype | References |
|---|---|---|
2EthOH− | leu2 | Ishchuk et al., 2008 [28] |
2EthOH−/XYL1m/XYL2 | pX1M-Z-X2 (GAPp-XYL1mod-AOXt, GAPp-XYL2-XYL2t) | This study |
2EthOH−/XYL1m/XYL2/XYL3 | pX1M-Z-X2 (GAPp-XYL1mod-AOXt, GAPp-XYL2-XYL2t), pGLG61/XYL3 (GAPp-XYL3-AOXt) | This study |
2EthOH−/XYL1m/XYL2/XYL3/BrPA | As above with unfixed mutations caused by selection on 3-BrPA containing medium | This study |
2EthOH−/XYL1m/XYL2/PDC1 | pX1M-Z-X2 (GAPp-XYL1mod-AOXt, GAPp-XYL2-XYL2t), pGLG61 + prGAP + PDC1Hp (GAPp-PDC1-AOXt) | This study |
2EthOH−/XYL1m/XYL2/XYL3/PDC1 | pX1M-Z-X2 (GAPp-XYL1mod-AOXt, GAPp-XYL2-XYL2t), pGLG61/XYL3/PDC1 (GAPp-XYL3-AOXt, GAPp-PDC1-AOXt) | This study |