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Table 2 P. pastoris genes with putative Aft1 binding site(s) related to the cellular secretory pathway machinery

From: Pichia pastoris Aft1 - a novel transcription factor, enhancing recombinant protein secretion

GO slim term - Biological process

Number (Genes)

GO:0006605 protein targeting

23 (ATG1 ATG12 ATG18 GET3 GET4 IMP2 KAR2 LHS1 NPL6 NUP188 NUP84 PAM17 PEX14 PEX7 PEX8 SEC61 SPC1 SPC3 TAM41 VPS21 VPS64 VPS68 VPS8)

GO:0048193 Golgi vesicle transport

19 (AGE2 APL4 CHS5 DRS2 EMP24 GET3 GOS1 HRR25 KES1 SEC12 SEC23 SFB3 SNC2 SVP26 TED1 TLG1 TRS31 VTI1 YPT32)

GO:0006979 response to oxidative stress

12 (AFT1 CTA1 EOS1 GCY1 GSH1 HMX1 HSP104 MXR1 POS5 TSA1 YAP1 YBL055C)

GO:0006457 protein folding

11 (CAJ1 CCT2 CCT6 CPR6 EGD2 ERV2 HLJ1 HSP104 PDI1 SIS1 TSA1)

GO:0006486 protein glycosylation

10 (ALG7 EOS1 KRE5 MNN11 OCH1 OST6 PMT1 PMT5 STT3 SVP26)

GO:0016050 vesicle organization

10 (EMP24 GOS1 SEC12 SEC23 SFB3 SNC2 TLG1 UBC1 VPS4 VTI1)

GO:0007033 vacuole organization

9 (ATG1 ATG12 ENO1 GYP7 RDI1 TPM2 VPS21 VTI1 YHC3)

GO:0051049 regulation of transport

9 (AKL1 ARG81 CUP9 FPK1 GEF1 SEC12 SEC23 SLG1 TUP1)

GO:0006887 exocytosis

6 (ARG81 KES1 KIN1 SNC2 TPM2 YPT32)

  1. Search field: -1000bp upstream of the P. pastoris GS115 coding sequences. Binding motifs: ScAft1 (ANTGCACCC) and ScAft2 (BRCACCCB). Gene hits were categorized into biological function GO terms using AmiGO GO Slimmer (Yeast GO slim set, [25]).