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Table 1 Putative P. pastoris Aft1 binding sites found in promoters of selected genes known to enhance protein secretion

From: Pichia pastoris Aft1 - a novel transcription factor, enhancing recombinant protein secretion

Gene Functionsa Aft1 UBS (bp) Sequence
PDI1 Protein disulfide isomerase, ER chaperone, formation of disulfide bonds -423b to -409 gatcacaCACCctct
KAR2 ER chaperone, mediating protein folding, UPR regulation -870b to -856 tcgtataCACCctca
-194b to -180 tgtaataCACCcttg
SSA4 HSP70 protein, co-translational protein-membrane targeting and translocation of nascent proteins into the ER -608c to -594 actcatgCACCctta
-342b to -328 catggaaCACCccat
KIN2 Serine/threonine kinase involved in exocytosis -177c to -163 ataactgCACCcaga
NCP1 P450 reductase, involved in lipid metabolism (ergosterol biosynthesis) -539c to -525 ggttttgCACCcagg
  1. aFunctions are derived for the S. cerevisiae homologs of the P. pastoris genes in the Saccharomyces Genome Database.
  2. bAft2 matrix.
  3. cAft1 matrix.
  4. Upstream binding sites (UBS): 5' → 3', -1000 to -1 A(0)TG. The core binding sequences, representing the highest conserved, consecutive positions are highlighted. TFBS analysis: MatInspector [18] using the search groups fungi and general core promoter elements.