Pichia pastoris Aft1 - a novel transcription factor, enhancing recombinant protein secretion

Table 1 Putative P. pastoris Aft1 binding sites found in promoters of selected genes known to enhance protein secretion

Gene	Functions^a	Aft1 UBS (bp)	Sequence
PDI1	Protein disulfide isomerase, ER chaperone, formation of disulfide bonds	-423^b to -409	gatcacaCACCctct
KAR2	ER chaperone, mediating protein folding, UPR regulation	-870^b to -856	tcgtataCACCctca
KAR2	ER chaperone, mediating protein folding, UPR regulation	-194^b to -180	tgtaataCACCcttg
SSA4	HSP70 protein, co-translational protein-membrane targeting and translocation of nascent proteins into the ER	-608^c to -594	actcatgCACCctta
SSA4		-342^b to -328	catggaaCACCccat
KIN2	Serine/threonine kinase involved in exocytosis	-177^c to -163	ataactgCACCcaga
NCP1	P450 reductase, involved in lipid metabolism (ergosterol biosynthesis)	-539^c to -525	ggttttgCACCcagg

^aFunctions are derived for the S. cerevisiae homologs of the P. pastoris genes in the Saccharomyces Genome Database.
^bAft2 matrix.
^cAft1 matrix.
Upstream binding sites (UBS): 5' → 3', -1000 to -1 A(0)TG. The core binding sequences, representing the highest conserved, consecutive positions are highlighted. TFBS analysis: MatInspector [18] using the search groups fungi and general core promoter elements.

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