Figure 3
From: Pichia pastoris Aft1 - a novel transcription factor, enhancing recombinant protein secretion
Characterization of the AFT1 promoter. (A) GFP expression levels under control of P GAP and P AFT1 . Cultivation in 96-well plate. RFU: relative fluorescence units, average of 87 P. pastoris clones. (B) GFP expression levels under control of P AFT1 using different cultivation conditions. Glucose: cultivation time 69h, 2% glucose batch, 3 times addition of glucose to 0.5%. Methanol: cultivation time 92h, 48h glucose batch (1%), 3 times addition of methanol to 0.5%. Derepressed: cultivation time 69h, 2% glucose batch, no additional supplementation.