Figure 1
From: Pichia pastoris Aft1 - a novel transcription factor, enhancing recombinant protein secretion
Aft1 protein sequence comparison. Amino acid sequences: S. cerevisiae Aft1 [NCBI protein: NP_011444] and Aft2 [NCBI protein:NP_015122], K. lactis Aft [NCBI protein:CAH00307], and P. pastoris Aft1 [NCBI protein:CCA37276] were aligned using ClustalW2 [37]. asterisk: indicates positions which have a single, fully conserved residue, colon: indicates conservation between groups of strongly similar properties; period: indicates conservation between groups of weakly similar properties; shaded grey: conserved residues (Leu99*, Leu102, Cys291 and Cys293 conferring iron sensitivity. Ser210 and Ser224 involved in Msn5 recognition and nuclear export. Cys143, Cys215, His239 and His241, which are suggested to be part of a WRKY-motif involved in zinc binding [39]). Differences are boxed and/or highlighted red; *numbering is based on ScAft1.