Figure 2

Itaconic acid production by the transformants of different genes. The transformants of the different genes were screened for itaconate production. Each transformant was grown in 50 ml IPM on a rotary shaker at 37°C for 76 hr in three individual flaks. The titers of itaconic acid produced were determined by HPLC. WT was represented by the solid red circle. *: statistically significant (ρ < 0.05, beneficial for itaconate production). ⋄: statistically significant (ρ < 0.05, adverse for itaconate production). ο: statistically non-significant (ρ > 0.05, no impact on itaconate production).