Table 1 Oligonucleotide sequences of the various primers applied for the construction of the vectors, and subsequent verification of vector sequences
Name | Sequence (5′-3′) | Reference |
|---|---|---|
EcoRI FW | ATTTCACAGAATTC CAGGTGCAGCTGCAGCAGTCT | This study |
NotI RV | ATTTCACAGCGGCCGC CCGTTTCAGCTCCAGCTT | This study |
NotI FW | ATTTCACAGCGGCCGC GAATTCAGGCGCC | This study |
SalI RV | ATTTCACAGTCGAC TTATTAATGGTGATGATGGTG | This study |
5′ AOX1 | GACTGGTTCCAATTGACAAGC | Life Technologies |
3′ AOX1 | GCAAATGGCATTCTGACATCC | Life Technologies |