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Figure 2 | Microbial Cell Factories

Figure 2

From: Efficient production of single-chain fragment variable-based N-terminal trimerbodies in Pichia pastoris

Figure 2

Characterization of purified trimerbodies. (A) Reducing SDS-PAGE of anti-CEA scFv-based N-terminal trimerbody (MFE-23N) purified from HEK-293 cells or P. pastoris. The functionality of purified MFE-23N trimerbodies is demonstrated by ELISA against plastic immobilized CEA (B) and by FACS on CEA and CEA+ tumor cells (C). The anti-CEA C6G9 mAb (IgG) was used as control.

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