Figure 2
From: Use of Ubp1 protease analog to produce recombinant human growth hormone in Escherichia coli
SDS-PAGE of the growth hormone samples from successive purification stages. (A) M, low molecular weight (LMW) protein marker (94, 67, 43, 30, 20.1, and 14.4 kDa); 1, the protein dissolved in DRCI buffer; 2, peak I obtained after purification on DEAE Sepharose FF column; 3, peak I obtained after purification on SP Sepharose FF column; 4, protein after renaturation; 5, sample after ammonium sulfate precipitation. (B) M, low molecular weight (LMW) protein marker (94, 67, 43, 30, 20.1, and 14.4 kDa); 1, growth hormone European standard; 2, Phenyl Sepharose FF column entry; 3, fraction I obtained after purification on Phenyl Sepharose FF column; 4, fraction II obtained after purification on Phenyl Sepharose FF column; 5, fraction III obtained after purification on Phenyl Sepharose FF column.