Table 8 Primers used for site-directed mutagenesis
Primer name | Sequence (5′-3′) |
|---|---|
P110S sense | GTGAATTGGGTAACGAATCCAACTGTCCTCACGC |
P110S antisense | GCGTGAGGACAGTTGGATTCGTTACCCAATTCAC |
S117L sense | CTGTCCTCACGCTGACTTGTCTGGTTACACCCAC |
S117L antisense | GTGGGTGTAACCAGACAAGTCAGCGTGAGGACAG |
C295Y sense | CTCCATTGTTGGTTCTTACGTCGGTAACAGAGCTG |
C295Y antisense | CAGCTCTGTTACCGACGTAAGAACCAACAATGGAG |
S110P sense | GGTAACGAACCCAACTGTC |
S110P antisense | GACAGTTGGGTTCGTTACC |
Y295C sense | TTGGTTCTTGCGTCGGTAAC |
Y295C antisense | GTTACCGACGCAAGAACCAA |
Sense BamHI | GGGGGGATCCATGTCTATCCCAGAAACTC |
Antisense BglII | CTTTAGATCTTTATTTAGAAGTGTCAACAACG |