Table 7 Plasmids used in the study
Plasmid | Construction background | Reference |
|---|---|---|
YEplacHXT (reference plasmid) | YEplac195, HXT7p-PGKt URA3 | [31] |
YEplacHXT-natADH1 | YEplacHXT, native ADH1 | This study |
YEplacHXT-mutADH1 | YEplacHXT, mutated ADH1 | [10] |
YEplacHXT-mutADH1-rev110 | YEplacHXT, mutated ADH1, P110S | This study |
YEplacHXT-mutADH1-rev117 | YEplacHXT, mutated ADH1, S117L | This study |
YEplacHXT-mutADH1-rev295 | YEplacHXT, mutated ADH1, C295Y | This study |
YEplacHXT-natADH1-m110 | YEplacHXT, native ADH1, S110P | This study |
YEplacHXT-natADH1-m295 | YEplacHXT, native ADH1, Y295C | This study |
YEplacHXT-natADH1-m110,295 | YEplacHXT, native ADH1, Y295C, S110P | This study |
YEplacHXT-mutADH1-rev110, 117 | YEplacHXT, mutated ADH1, P110S, S117L | This study |