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Table 5 In vitro and in vivo conversion rate of HMF and specific growth, glucose consumption and ethanol production rates in anaerobic batch fermentation in defined mineral medium supplemented with 2 g/l HMF

From: Furaldehyde substrate specificity and kinetics of Saccharomyces cerevisiae alcohol dehydrogenase 1 variants

Strain

In vitro HMF reduction a (g/g cells. h)

In vivo conversion rate (g/g cells.h)

μ (1/h)

q glucose (g/g cells. h)

q ethanol (g/g cells. h)

Control

ndb

0.14 ± 0.00

0.07 ± 0.00

1.54 ± 0.09

0.58 ± 0.06

CEN.PK 113-5D [mutAdh1p]

2.57 ± 0.32

0.47 ± 0.03

0.13 ± 0.01

2.96 ± 0.63

1.19 ± 0.09

CEN.PK 113-5D [natAdh1p-m110, 295]

17.81 ± 2.68

0.48 ± 0.00

0.13 ± 0.01

3.08 ± 0.70

1.17 ± 0.16

  1. aThe in vitro activities for these strains were recalculated as gHMF converted per gram of cells per hour. This allowed the comparison between the conversion rates during the fermentation and the in vitro measurements. bNot detected.
  2. Rates were calculated during exponential growth. Presented values are the mean of two independent biological replicates.
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Microbial Cell Factories

ISSN: 1475-2859

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