Table 2 Strains used in the study
S. cerevisiae strains | Relevant genotype | Reference |
|---|---|---|
CEN.PK 113-5D | Mata ura3 | [16] |
Control (reference plasmid) | CEN.PK 113-5D, YEplacHXT | [10] |
CEN.PK 113-5D [natAdh1p] | CEN.PK 113-5D, YEplacHXT-natADH1 | This study |
CEN.PK 113-5D [mutAdh1p] | CEN.PK 113-5D, YEplacHXT-mutADH1 | [10] |
CEN.PK 113-5D [mutAdh1p-rev110] | CEN.PK 113-5D, YEplacHXT-mutADH1-rev110 | This study |
CEN.PK 113-5D [mutAdh1p-rev117] | CEN.PK 113-5D, YEplacHXT-mutADH1-rev117 | This study |
CEN.PK 113-5D [mutAdh1p-rev295] | CEN.PK 113-5D, YEplacHXT-mutADH1-rev295 | This study |
CEN.PK 113-5D [natAdh1p-m110] | CEN.PK 113-5D, YEplacHXT-natADH1-m110 | This study |
CEN.PK 113-5D [natAdh1p-m295] | CEN.PK 113-5D, YEplacHXT-natADH1-m295 | This study |
CEN.PK 113-5D [natAdh1p-m110, 295] | CEN.PK 113-5D, YEplacHXT-natADH1-m110, 295 | This study |
CEN.PK 113-5D [mutAdh1p-rev110, 117] | CEN.PK 113-5D, YEplacHXT-mutADH1-rev110, 117 | This study |
BY4741 | Mat-a his3Δ1 leu2Δ0 met15Δ0 ura3Δ0, deleted ORF YOL086C (adh1) | EUROSCARF collection, Heidelberg, Germany |
BY4741 [natAdh1p] | BY4741, YEplacHXT-natADH1 | This study |
BY4741 [mutAdh1p] | BY4741, YEplacHXT-mutADH1 | This study |
BY4741 [mutAdh1p-rev117] | BY4741, YEplacHXT-mutADH1-rev117 | This study |
BY4741 [natAdh1p-m110,295] | BY4741, YEplacHXT-natADH1-m110, 295 | This study |
BY4741 [mutAdh1p-rev110,117] | BY4741, YEplacHXT-mutADH1-rev110, 117 | This study |