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Table 3 Growth, nitrogen consumption, and nitrogen fate for four strains at the end of nitrogen consumption and for two strains at the point of 50% nitrogen consumption in SM260

From: Efficient ammonium uptake and mobilization of vacuolar arginine by Saccharomyces cerevisiae wine strains during wine fermentation

  

50% YAN consumption

End of YAN consumption

  

EC1118

YPS128

WE372

EC1118

YPS128

YPS1009

Population

106 cell.mL−1

45.5 ± 1.2

51.8 ± 1.7

135.2 ± 0.1

129.8 ± 1.0

109.2 ± 3.1

112.0 ± 1.2

Vol

μm3

73.2 ± 0.5

63.3 ± 1.0

56.3 ± 0.5

61.6 ± 0.2

74.4 ± 1.0

67.4 ± 1.4

DW

g.L−1

1.55 ± 0.02

1.44 ± 0.02

3.20 ± 0.02

3.20 ± 0.03

2.70 ± 0.01

2.70 ± 0.12

YANc

       

Total

mgN.L−1

160 ± 1

143 ± 1

258 ± 1

258 ± 1

258 ± 1

258 ± 1

NH4+

mgN.L−1

60.4 ± 0.3

46.9 ± 0.7

61.5 ± 0.4

61.5 ± 0.4

61.5 ± 0.4

61.5 ± 0.4

Arg

mgN.L−1

20.4 ± 0.3

20.0 ± 0.4

78.5 ± 0.5

78.5 ± 0.5

78.5 ± 0.5

78.5 ± 0.5

Proteins

       
 

% (g.g−1)

56.9 ± 0.3

56.5 ± 0.4

47.6 ± 0.4

47.3 ± 0.2

46.0 ± 0.1

44.8 ± 0.7

 

mgN.L−1

142 ± 2

130 ± 1

247 ± 0

245 ± 2

202 ± 2

192 ± 0

Vacuole

       

Total

mgN.L−1

9.5 ± 0.1

6.3 ± 0.3

6.7 ± 0.2

9.4 ± 0.2

20.2 ± 0.7

23.7 ± 0.3

Arg

mgN.L−1

3.7 ± 0.2

4.1 ± 0.1

1.7 ± 0.0

2.9 ± 0.1

10.7 ± 0.3

8.4 ± 0.2

Cytoplasm

       

Total

mgN.L−1

4.7 ± 0.0

3.9 ± 0.1

6.4 ± 0.2

5.3 ± 0.1

5.0 ± 0.1

3.6 ± 0.1

  1. PopM: maximal cellular population (106 cell .mL−1), Vol: cellular volume (μm3), DW: dry weight (g.L−1), YANc: yeast assimilable nitrogen consumed (mg N.L−1), YDW/YAN: yield of biomass production from nitrogen consumed (g.g−1), Proteins: percentage of proteins (g.L−1) divided by the dry weight (g.L−1) (%) or amount of nitrogen found in proteins (mg N.L−1), Vacuole: amount of nitrogen in the vacuole, Total (mg N.L−1) and as Arginine (mg N.L−1), and Cytoplasm: amount of nitrogen found in the cytoplasm (mg N.L−1).
  2. Mean values and standard errors were calculated from three replicates (except for strains WE372 and YPS1009 for which only duplicates were used).