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Table 2 Fermentative capacity, growth and nitrogen consumption traits of six S. cerevisiae strains during fermentations containing various nitrogen concentrations

From: Efficient ammonium uptake and mobilization of vacuolar arginine by Saccharomyces cerevisiae wine strains during wine fermentation

Media

Strains

DW

r YANmax

T 50

CO 2F

r CO2max

Y DW/YAN

  

g.L −1

mg N.L −1 .h −1

h

g.L −1

g.L −1 .h −1

g.g −1

SM45

EC1118

0.9 ± 0.01

5.9 ± 0.86

14.1 ± 0.71

110 (na)

0.4 ± 0.03

20.7 ± 0.16

 

L2226

nd

6.3 (na)

15.5 (na)

nd

0.5 ± 0.01

nd

 

WE372

nd

4.8 ± 0.57

15.0 (na)

nd

0.4 ± 0.01

nd

 

NCYC110

0.6 ± 0

4.8 ± 0.41

22.3 ± 0.32

90 (na)

0.5 ± 0.04

14.5 ± 0.01

 

YPS1009

nd

3.9 ± 0.08

17.5 (na)

nd

0.4 ± 0.01

nd

 

YPS128

0.9 ± 0.0

6.7 ± 0.33

18.0 (na)

102 (na)

0.4 ± 0.01

21.3 ± 0.32

SM85

EC1118

1.6 ± 0.02

10.6 ± 0.60

15.5 ± 0.14

111 (na)

0.9 ± 0.01

19.6 ± 0.25

 

L2226

1.7 (na)

11.3 (na)

15.0 (na)

111 (na)

1.0 (na)

20.9 (na)

 

WE372

1.9 (na)

11.6 (na)

nd

111 (na)

1.1 (na)

22.2 (na)

 

NCYC110

1.1 (na)

8.6 (na)

22.7 (na)

86 (na)

0.9 (na)

13.5 (na)

 

YPS1009

1.3 (na)

8.8 (na)

17.9 (na)

99 (na)

0.9 (na)

15.9 (na)

 

YPS128

1.3 ± 0.02

9 ± 0.53

18.4 ± 0.42

104 (na)

0.8 ± 0.01

15.5 ± 0.30

SM165

EC1118

3.2 ± 0.04

24.3 ± 0.08

16.6 ± 0.14

112 ± 1.4

2.8 ± 0.08

19.4 ± 0.22

 

L2226

3.2 ± 0.18

27.8 ± 0.44

15.3 ± 0.85

113 ± 0.1

2.5 ± 0.22

19.5 ± 1.12

 

WE372

3.4 ± 0.15

24.4 ± 0.08

15.1 ± 0.07

115 ± 3.2

2.3 ± 0.07

21.1 ± 0.92

 

NCYC110

2.1 ± 0.14

16.8 ± 0.79

22.6 ± 0.28

97 ± 2.6

2.1 ± 0.07

13.0 ± 0.87

 

YPS1009

2.4 ± 0.14

21.1 ± 0.19

17.5 ± 0.28

99 ± 2.3

2.0 ± 0.04

14.7 ± 0.87

 

YPS128

2.4 ± 0.18

21.1 ± 1.12

18.1 ± 0.14

105 ± 0.8

1.7 ± 0.07

14.7 ± 1.12

SM260

EC1118

3.4 ± 0.12

30.5 (na)

17.0 (na)

113 (na)

2.5 ± 0.28

13.2 ± 0.48

 

L2226

3.5 (na)

31.6 (na)

15.5 (na)

112 (na)

2.5 (na)

13.5 (na)

 

WE372

3.8 (na)

31.5 (na)

16.5 (na)

111 (na)

2.4 (na)

14.7 (na)

 

NCYC110

2.8 (na)

20.8 (na)

21.2 (na)

101 (na)

2.4 (na)

10.8 (na)

 

YPS1009

2.8 ± 0.09

25.1 ± 0.16

20.0 ± 0.14

102 ± 0.1

2.2 ± 0.23

10.9 ± 0.31

 

YPS128

2.8 ± 0.12

24.9 (na)

18.0 (na)

105 (na)

2.1 ± 0.16

11.1 ± 0.48

SM385

EC1118

4.6 ± 0.08

30.2 ± 0.15

20.5 ± 0.21

113 ± 0.9

2.7 ± 0.04

14.4 ± 0.27

 

L2226

5.3 ± 0.04

40.9 ± 0.03

18.7 (na)

110 ± 0.0

3.4 ± 0.01

14.6 ± 0.10

 

WE372

5.1 ± 0.01

31.6 ± 0.01

20.5 (na)

110 ± 0.0

2.8 ± 0.01

15.8 ± 0.46

 

NCYC110

3.9 ± 0.31

18.4 ± 0.67

25.5 (na)

111 ± 1.9

2.9 ± 0.07

12.7 ± 0.34

 

YPS1009

3.4 ± 0.12

24.5 ± 0.02

22.5 (na)

108 ± 1.1

2.1 ± 0.01

13.3 ± 0.46

 

YPS128

3.5 ± 0.11

20.6 ± 1.02

23 ± 0.42

105 ± 6.6

2.1 ± 0.03

12.7 ± 0.59

 

Type

10.86***

8.44***

42.73***

53.91***

2.96***

32.57***

 

Media

85***

82.77***

34**

12.65

91.86***

50.78***

 

Type by Media

2.34**

4.69 **

0.43

9.71

1.65

5.77

 

Residual

1.8

4.1

22.85

23.73

3.54

10.88

 

Adj.R squared a

97

94

66

65

95

83

  1. DW: dry weight, rXmax: maximal growth rate, T50: time at which 50% of YAN was consumed, rYANmax: maximal rate of YAN consumption, CO2F: total amount of CO2 released, rCO2max: maximal CO2 production rate, and YDW/YAN: yield of biomass production with respect to consumed nitrogen. Mean values and standard errors were calculated from two replicates. Two-way ANOVA was carried out on each variable: Fixed factors were the type of strain (high or low), and the media (SM45, SM85, SM165, SM260, or SM385), and their interaction. A p-value threshold of 0.05 was considered significant.
  2. na: not applicable (missing replicate); nd: not determined (not measured).
  3. aAjusted R square (%).
  4. **, significant at p-value < 0.01, ***, significant at p-value < 0.001.