Figure 3

Comparison of the effects of signal peptides on itaconate production from liquefied corn starch. WT and the transformants XH61-5 and XH86-8 were compared in the one-step (A) and two-step (B) processes using liquefied corn starch (equivalent to 140 g/L glucose) as the carbon source. Cultures were sampled every 12 h. The itaconate titers were determined by HPLC. Itaconic acid production from saccharified corn starch hydrolysate (SCSH) by WT was used as the reference.