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Table 6 Plasmids used in the study

From: L-lactic acid production from D-xylose with Candida sonorensis expressing a heterologous lactate dehydrogenase encoding gene

Plasmid

Description

Reference or source

pCM29

ScP PDC1 -hph-ScT GAL10 -XYLA

C. Miller, NatureWorks LLC

pVR103

ScP TEF -ScXKS1-ScT GAL10

V. Rajgarhia, NatureWorks LLC

pMI271

CsP TDH1 -hph-ScT GAL10

[6]

pMI278

CsP GPD1 -G418 R -ScT MEL5 -CsPPGK1-BmLDH-ScT GAL10

[6]

pMI281

CsP TDH1 -BmLDH-ScT GAL10

This study

pMI400

ScP PDC1 -hph-ScT GAL10 -XYLA

This study

pMI403

CsP TDH1 -G418 R -ScT MEL5 -CsP PGK1 -XYLA-ScT GAL10

This study

pMI406

ScXKS1-ScT GAL10

This study

pMI409

CsXYL2 5′-CsP TDH1 -BmLDH-ScT GAL10

This study

pMI410

CsXYL2 5′-CsP TDH1 -BmLDH-ScT GAL10 -CsXYL2 3′

This study

pMI411

CsXYL1 5′-CsP TDH1 -BmLDH-ScT GAL10

This study

pMI412

CsXYL1 5′-CsP TDH1 -BmLDH-ScT GAL10 -CsXYL1 3′

This study

pMI417

CsXYL1 5′-CsP TDH1 -G418 R -ScT MEL5 -CsP PGK1 -XYLA-ScT GAL10 -CsXYL1 3′

This study

pMI423

CsP PGK1 -XYLA-ScT GAL10 -CsP TDH1 -hph-ScT GAL10

This study

pMI424

CsXYL1 5′-CsP TDH1 -G418 R -ScT MEL5 -CsP PGK1 -ScXKS1-ScT GAL10 -CsXYL1 3′

This study

pMI425

CsXYL2 5′-CsP TDH1 -hph-ScT MEL5 -CsP PGK1 -ScXKS1-ScT GAL10 -CsXYL2 3′

This study

pKK02

CsXYL2 5′-CsP TDH1 -hph-ScT MEL5 -CsXYL2 3′

This study

pKK03

CsXYL1 5′-CsP TDH1 -G418 R -ScT MEL5 -CsXYL1 3′

This study