L-lactic acid production from D-xylose with Candida sonorensis expressing a heterologous lactate dehydrogenase encoding gene

Table 3 L-lactic acid, xylitol and D-xylulose production with XYLA strains in microaerobic cultivation

Strains	L-lactic acid (g l ⁻¹ )	L-lactic acid yield (g g ⁻¹ )	Xylitol (g l ⁻¹ )	D-Xylulose (g l ⁻¹ )
C29	31.0 ± 0.3	0.59 ± 0.01	8.1 ± 0.3*	0.43 ± 0.01
C281	30.9 ± 0.3	0.60 ± 0.01	8.7 ± 0.2*	0.63 ± 0.02*
C282-C284	31.8 ± 0.1*	0.61 ± 0.00	6.7 ± 0.2	0.45 ± 0.00

Concentrations (g l⁻¹) of L-lactic acid, xylitol, and D-xylulose produced and yield of L-lactic acid on D-xylose consumed (g g⁻¹) by C. sonorensis ldhL strains in which the native D-xylose pathway was intact (C29, C281 and C282-C284). All strains except C29 expressed XYLA alone (C281) or with ScXKS1 (C282-C284). Cells were cultivated in CaCO₃ buffered, YP medium with 50 g l⁻¹ D-xylose and 10 mM MgCl₂ at 30°C, 100 rpm. Results are the mean ± sem from 3 to 6 replicate flasks. An asterisk indicates that the value is significantly different (p < 0.05) from values in the same column without an asterisk.

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