L-lactic acid production from D-xylose with Candida sonorensis expressing a heterologous lactate dehydrogenase encoding gene

Table 2 Xylose isomerase activity

Xylose isomerase (XI) activity in C. sonorensis C29 containing ldhL, and in ldhL strains expressing the Piromyces sp. XYLA gene alone (C281) or with the S. cerevisiae XKS1 gene (C282-C284). The activity was measured from cell free extracts prepared after overnight cultivation of cells in YP with 50 g l⁻¹ D-glucose and 10 mM MgCl₂. The activity values are means ± sem from 3 (C29, C281) or 6 (C282-C284, duplicates of three independent transformants) aerobic flask cultivations.

ISSN: 1475-2859