L-lactic acid production from D-xylose with Candida sonorensis expressing a heterologous lactate dehydrogenase encoding gene

Table 1 Maximum L-lactic acid and xylitol production in microaerobic cultivation

Strain	Genotype	L-lactic acid (g l ⁻¹ )	L-lactic acid yield (g g ⁻¹ )	Xylitol (g l ⁻¹ )
Wild type¹		n.d.	n.d.	7.8 ± 1.1*
C29	x::ldhL	26.7 ± 0.8	0.53 ± 0.03	4.6 ± 0.5
C184	pdc1Δ::ldhL pdc2Δ	27.3 ± 0.8	0.53 ± 0.02	3.9 ± 0.7
C169	pdc1Δ::ldhL pdc2Δ::ldhL	30.8 ± 1.0*	0.57 ± 0.03	5.6 ± 0.5

¹The wild type strain also produced 4.0 ± 0.9 g ethanol l⁻¹ and 6.5 ± 0.9 g acetate l⁻¹.
Maximum concentrations of L-lactic acid and xylitol produced from D-xylose by C. sonorensis wild type and C29, C169 and C184, and yield of L-lactic acid from D-xylose. Cells were cultivated in buffered YNB medium with 50 g l⁻¹ D-xylose medium at 30°C, 100 rpm. Data are mean ± sem from 3 to 12 replicate cultures. n.d. = not detected. An asterisk indicates that the value is significantly different (p < 0.05) from other values in the same column.

ISSN: 1475-2859