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Figure 2 | Microbial Cell Factories

Figure 2

From: Ag43-mediated display of a thermostable β-glucosidase in Escherichia coli and its use for simultaneous saccharification and fermentation at high temperatures

Figure 2

SDS-PAGE showing the protease accessibility of BglC attached to the surface of the outer membrane of E. coli MS04. PM refers to the protein marker; P106 is the protein product of pAg43BglC without the SP and represents the sum of Ag43 TU and BglC. The plasmid pTrc99A2 was employed as a negative control. The (−) symbol in the pAg43BglC line means that the proteins were from cultures grown with MS04/pTrc99A2. The (−) symbol in the trypsin line means OMPs of cells not treated with trypsin. Note that OmpC, which migrates together with OmpF, is not found in the figure because during the development of strain MS04, an adaptive evolution step to improve the consumption and growth on xylose was developed, and a region of the genome that contains the ompC gene was lost [5],[28].

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