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Table 4 Results of analysis of DNA sequence of the insert in clone 129–3

From: The use of fosmid metagenomic libraries in preliminary screening for various biological activities

ORF position in the clone

Putative gene

Significant similarity (%) to known gene from indicated organism

Description

1885-3162

thrC

73% Starkeya novella DSM 506

COG0498 Threonine synthase [amino acid transport and metabolism]

4778-5017

rimL

70% Polymorphum gilvum SL003B-26A1

COG1670 Acetyltransferases, including N-acetylases of ribosomal proteins [Ttanslation, ribosomal structure and biogenesis]

10222-8198

phaS

74% Polymorphum gilvum SL003B-26A1

TIGR01838 poly (R)-hydroxyalkanoic acid synthase, class I [fatty acid and phospholipid metabolism, Biosynthesis]

11838-13154

homD

69% Polymorphum gilvum SL003B-26A1

PRK06349 Homoserine dehydrogenase [synthesis of Met, Thr, Ile from Asp]

19761-21452

arnT

78% Starkeya novella DSM 506

COG1807 4-amino-4-deoxy-L-arabinose transferase and related glycosyltransferases of PMT family [amino acids and vitamins transport]

22371-22703

lpxB

76% Mesorhizobium loti MAFF303099

PRK01021 lipid-A-disaccharide synthase

23930-22725

metC

65% Starkeya novella DSM 506

PRK05967 cystathionine beta-lyase [methionine and cysteine metabolism]

24090-25163

hisJ

67% Starkeya novella DSM 506

COG0834 ABC-type amino acid transport/signal transduction systems, periplasmic component/domain [amino acid transport and metabolism/signal transduction mechanisms]

29290-27989

cfaS

70% Mesorhizobium opportunistum WSM2075

COG2230 Cyclopropane fatty acid synthase and related methyltransferases [cell envelope biogenesis, outer membrane]

  1. Selected genes located in this DNA fragment are described.