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Table 3 Results of analysis of DNA sequence of the insert in clone 123–3

From: The use of fosmid metagenomic libraries in preliminary screening for various biological activities

ORF position in the clone

Putative gene

Significant similarity (%) to known gene from indicated organism

Description

401-1672

gckA

Not found

COG2379 Putative glycerate kinase [carbohydrate transport and metabolism]

2611-1691

phpP

Not found

PLN02645 phosphoglycolate phosphatase

4201-2720

gatA

76% Rhizobium sp. strain NT-26

COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases [translation, ribosomal structure and biogenesis]

4976-4689

gatC

76% Rhizobium sp. strain NT-26

COG0721 Asp-tRNAAsn/Glu-tRNAGln amidotransferase C subunit [Translation, ribosomal structure and biogenesis]

5828-5118

medH

75% Agrobacterium vitis S4

PRK00685 metal-dependent hydrolase; Provisional

11125-12420

pyrC

67% Rhizobium sp. strain NT-26

cd01317 Dihydroorotase (DHOase), subgroup IIa; DHOases catalyze the reversible interconversion of carbamoyl aspartate to dihydroorotate, a key reaction in pyrimidine biosynthesis

20911-22095

matA

71% Rhizobium leguminosarum bv. trifolii CB782

Multidrug ABC transporter ATPase/protein_id = “AHG44931.1

22188-22355

rpmG

71% Rhizobium sp. str. NT-26

PRK00595 50S ribosomal subunit protein L33

  1. Selected genes located in this DNA fragment are described.