Skip to main content

Table 6 Plasmids used in this study

From: Metabolic engineering of Escherichia coli for the production of riboflavin

Plasmid Characteristics a Source
pUC18-SVG pUC18 PvegI-gnd361, Ampr, Spcr [13]
pUC18-SVZ pUC18 PvegI-zwf243, Ampr, Spcr [13]
p5C Expression vector, pSC101 replication, Ptrc, Ampr Lab collection
p15C Expression vector, p15A replication, Ptrc, Ampr Lab collection
p20C Expression vector, pBR322 replication, Ptrc, Ampr Lab collection
p5C-EC10 pSC101 replication, Ampr, Ptrc-synRBS-ribA-synRBS-ribB- synRBS-ribD-synRBS-ribE-synRBS-ribC This study
p15C-EC10 p15A replication, Ampr, Ptrc-synRBS-ribA-synRBS-ribB-synRBS- ribD-synRBS-ribE-synRBS-ribC This study
p20C-EC10 pBR322 replication, Ampr, Ptrc-synRBS-ribA-synRBS-ribB- synRBS-ribD-synRBS-ribE-synRBS-ribC This study
p20C-BS15 pBR322 replication, Ampr, Ptrc-synRBS-ribB/A-synRBS-ribD- synRBS-ribE-synRBS-ribC This study
p20C-BSrib pBR322 replication, Ampr, Ptrc-BSrib operon This study
p15Trc Expression vector, p15A replication, Cmr Lab collection
p15Trc -zg p15A replication, Cmr, Ptrc-synRBS-zwf-synRBS-gnd, zwf and gnd genes from C. glutamicum This study
p15Trc -zgp p15A replication, Cmr, Ptrc-synRBS-zwf-synRBS-gnd-synRBS-pgl, zwf and gnd genes from C. glutamicum, pgl gene from E. coli This study
pTKS/CS p15A replication, Cmr, Tetr, I-SceI restriction sites [52]
pTKRED pSC101 replication, temperature sensitive replication origin, Spcr, ParaBAD-driven I-SceI gene, Red recombinase expression plasmid, lac-inducible expression [52]
  1. a Abbreviations: Amp ampicillin, Cm chloramphenicol, Km kanamycin, Tet tetracycline, r resistance.