Table 6 Plasmids used in this study

pUC18-SVG

pUC18 P_vegI-gnd361, Amp^r, Spc^r

[13]

pUC18-SVZ

pUC18 P_vegI-zwf243, Amp^r, Spc^r

[13]

p5C

Expression vector, pSC101 replication, Ptrc, Amp^r

Lab collection

p15C

Expression vector, p15A replication, Ptrc, Amp^r

Lab collection

p20C

Expression vector, pBR322 replication, Ptrc, Amp^r

Lab collection

p5C-EC10

pSC101 replication, Amp^r, P_trc-synRBS-ribA-synRBS-ribB- synRBS-ribD-synRBS-ribE-synRBS-ribC

This study

p15C-EC10

p15A replication, Amp^r, P_trc-synRBS-ribA-synRBS-ribB-synRBS- ribD-synRBS-ribE-synRBS-ribC

This study

p20C-EC10

pBR322 replication, Amp^r, P_trc-synRBS-ribA-synRBS-ribB- synRBS-ribD-synRBS-ribE-synRBS-ribC

This study

p20C-BS15

pBR322 replication, Amp^r, P_trc-synRBS-ribB/A-synRBS-ribD- synRBS-ribE-synRBS-ribC

This study

p20C-BSrib

pBR322 replication, Amp^r, P_trc-BSrib operon

This study

p15Trc

Expression vector, p15A replication, Cm^r

Lab collection

p15Trc -zg

p15A replication, Cm^r, P_trc-synRBS-zwf-synRBS-gnd, zwf and gnd genes from C. glutamicum

This study

p15Trc -zgp

p15A replication, Cm^r, P_trc-synRBS-zwf-synRBS-gnd-synRBS-pgl, zwf and gnd genes from C. glutamicum, pgl gene from E. coli

This study

pTKS/CS

p15A replication, Cm^r, Tet^r, I-SceI restriction sites

[52]

pTKRED

pSC101 replication, temperature sensitive replication origin, Spc^r, P_araBAD-driven I-SceI gene, Red recombinase expression plasmid, lac-inducible expression

[52]