Skip to main content

Table 4 Riboflavin production of RF05S-M40 in different cultural conditions

From: Metabolic engineering of Escherichia coli for the production of riboflavin

Medium

Glucose (g/L)

Temperature (°C)

Yeast extract (g/L)

OD600 a

Biomass b (g/L)

Riboflavin (mg/L)

Riboflavin yield (mg/g) c

Specific productivity (mg-RF/g-DCW/h)

M9

10d

37

1

4.1±0.2

1.6±0.1

271.8±4.8

66.4±8.6

3.6±0.2

M9

37

3

7.0±0.1

2.7±0.1

289.5±7.9

25.2±0.8

2.3±0.1

M9

37

5

6.8±0.1

2.7±0.1

532.4±24.9

50.7±0.6

4.3±0.2

MS

37

1

6.4±0.1

2.4±0.1

702.4±17.4

66.0±0.7

6.1±0.2

MS

37

3

6.3±0.2

2.4±0.1

811.2±43.8

79.7±2.3

7.1±0.5

MS

37

5

7.7±0.3

2.9±0.1

847.4±38.0

82.7±3.2

6.1±0.4

M9

31

5

7.7±0.2

2.9±0.1

892.5±23.1

84.3±3.8

6.4±0.3

MS

31

1

6.5±0.2

2.1±0.1

577.6±11.4

66.5±1.9

4.9±0.2

MS

31

3

7.3±0.2

2.8±0.1

907.7±12.8

93.1±2.3

6.8±0.2

MS

31

5

7.6±0.2

2.9±0.1

1204.1±40.4

120.6±5.4

8.7±0.4

  1. aOD600 was the final optical density of the culture.
  2. bThe average cell dry weight for all of the strains was 0.38 g/L per optical-density (OD600) unit of culture.
  3. cmg/g: mg riboflavin/g-consumed glucose.
  4. dBatch cultures of RF05S-M40 was carried out in modified M9 or MS containing 10 g/L glucose at 31°C or 37°C. Standard deviations were calculated from the results of three independent experiments.
Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us