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Table 4 Riboflavin production of RF05S-M40 in different cultural conditions

From: Metabolic engineering of Escherichia coli for the production of riboflavin

Medium Glucose (g/L) Temperature (°C) Yeast extract (g/L) OD600 a Biomass b (g/L) Riboflavin (mg/L) Riboflavin yield (mg/g) c Specific productivity (mg-RF/g-DCW/h)
M9 10d 37 1 4.1±0.2 1.6±0.1 271.8±4.8 66.4±8.6 3.6±0.2
M9 37 3 7.0±0.1 2.7±0.1 289.5±7.9 25.2±0.8 2.3±0.1
M9 37 5 6.8±0.1 2.7±0.1 532.4±24.9 50.7±0.6 4.3±0.2
MS 37 1 6.4±0.1 2.4±0.1 702.4±17.4 66.0±0.7 6.1±0.2
MS 37 3 6.3±0.2 2.4±0.1 811.2±43.8 79.7±2.3 7.1±0.5
MS 37 5 7.7±0.3 2.9±0.1 847.4±38.0 82.7±3.2 6.1±0.4
M9 31 5 7.7±0.2 2.9±0.1 892.5±23.1 84.3±3.8 6.4±0.3
MS 31 1 6.5±0.2 2.1±0.1 577.6±11.4 66.5±1.9 4.9±0.2
MS 31 3 7.3±0.2 2.8±0.1 907.7±12.8 93.1±2.3 6.8±0.2
MS 31 5 7.6±0.2 2.9±0.1 1204.1±40.4 120.6±5.4 8.7±0.4
  1. aOD600 was the final optical density of the culture.
  2. bThe average cell dry weight for all of the strains was 0.38 g/L per optical-density (OD600) unit of culture.
  3. cmg/g: mg riboflavin/g-consumed glucose.
  4. dBatch cultures of RF05S-M40 was carried out in modified M9 or MS containing 10 g/L glucose at 31°C or 37°C. Standard deviations were calculated from the results of three independent experiments.