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Table 3 Riboflavin production of the various strains constructed in the study

From: Metabolic engineering of Escherichia coli for the production of riboflavin

Strain

OD600 a

Biomass (g/L) b

Riboflavin (mg/L)

Riboflavin yield (mg/g) c

Specific productivity (mg-RF/g-DCW/h)

RF02S

5.4±0.3

2.0±0.1

394.8±9.6

39.9±1.1

5.1±0.2

RF03S

5.2±0.4

1.9±0.1

559.9±8.9

59.9±0.8

8.2±0.3

RF05S

4.9±0.2

1.8±0.1

585.2±13.6

59.3±1.3

8.6±0.2

  1. aOD600 is the final optical density of the culture.
  2. bThe average cell dry weight for all of the strains was 0.38 g/L per optical-density (OD600) unit of culture.
  3. cmg/g: mg riboflavin/g-consumed glucose.
  4. Batch cultures of recombinant strains were carried out in LBG medium (LB with 10 g/L glucose) at 37°C as described in methods. Standard deviations were calculated from the results of three independent experiments.
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Microbial Cell Factories

ISSN: 1475-2859

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