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Table 1 Activities of GTP cyclohydrolase II and Riboflavin synthase

From: Metabolic engineering of Escherichia coli for the production of riboflavin

Strain/Plasmid

Enzyme activities (nmol/min/mg protein)

GTP cyclohydrolase II a

Riboflavin synthase b

E. coli MG1655/p20C-EC10

8.6±0.4

14.3±1.6

E. coli MG1655/p20C-BSrib

6.4±0.3

10.9±0.4

E. coli MG1655/p20C-BS15

6.3±0.2

9.6±0.4

  1. aOne unit of enzyme activity catalyzes the formation of 1 nmol of 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate per min.
  2. bOne unit of enzyme activity catalyzes the formation of 1 nmol of riboflavin per min.
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Microbial Cell Factories

ISSN: 1475-2859

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