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Table 1 Box-Behnken matrix representing real levels of operational parameters.

From: Development of a two-step cultivation strategy for the production of vitamin B12 by Bacillus megaterium

 

Sample no.

A: Step 1 + O2Hours

B: Step 2 -O2Hours

C: Step 3 + O2Hours

Run

1

8

18

15

18

2

14

9

15

12

3

15

9

15

12

4

1

0

6

12

5

6

18

15

6

6

11

9

6

18

7

3

0

24

12

8

5

0

15

6

9

17

9

15

12

10

13

9

15

12

11

12

9

24

18

12

10

9

24

6

13

4

18

24

12

14

9

9

6

6

15

7

0

15

18

16

16

9

15

12

17

2

18

6

12