Skip to main content

Table 1 Metabolic characterizations of B. subtilis strains in minimal medium supplemented with 20 g/L glucose

From: Deregulation of purine pathway in Bacillus subtilis and its use in riboflavin biosynthesis

Strains

BS77

BS89

BS93

BS96

BS102

BS103

BS104

BS106

BS107

BS110

BS111

Specific growth rate (h-1)

0.66 ± 0.01

0.64 ± 0.00

0.59 ± 0.01

0.62 ± 0.00

0.61 ± 0.01

0.61 ± 0.01

0.56 ± 0.01

0.60 ± 0.01

0.55 ± 0.01

0.45 ± 0.01

0.64 ± 0.04

Specific riboflavin production rate (μmol•g-1CDW•h-1)

32.85 ± 2.66

41.65 ± 1.51

30.80 ± 2.72

6.52 ± 1.79

32.74 ± 1.81

35.10 ± 1.21

34.96 ± 1.58

51.46 ± 3.58

27.15 ± 2.04

32.79 ± 0.48

61.15 ± 0.85

Specific glucose uptake rate (mmol•g-1CDW•h-1)

18.82 ± 2.08

16.25 ± 0.96

17.02 ± 1.02

19.45 ± 1.54

16.13 ± 0.13

17.35 ± 1.06

14.33 ± 0.26

15.38 ± 0.39

12.35 ± 0.54

8.69 ± 0.19

18.94 ± 3.00

Riboflavin yield (mg/g glucose)

2.10 ± 0.02

5.02 ± 0.06

4.26 ± 0.20

1.10 ± 0.04

5.21 ± 0.07

4.97 ± 0.14

5.56 ± 0.45

6.55 ± 0.50

5.12 ± 0.05

8.46 ± 0.30

5.84 ± 0.04

  1. Bacteria were cultivated with 50 mL minimal medium in a 500 mL flask shaking at 240 rpm and 41°C; data were the average values of three parallel experiments.