Figure 4From: Antisense knockdown of pyruvate dehydrogenase kinase promotes the neutral lipid accumulation in the diatom Phaeodactylum tricornutum Confocal images of P. tricornutum cells stained with Nile red. Cells were photographed under confocal microscope at day 7 in the stationary phase. (A) wild type; (B) Transgenic line PtPDK-1. Left panel: fluorescence of neutral lipids; right panel: DIC (differential interference contrast). Bars = 5 μm. (C) The fluorescence of triplicate Nile red stained microalgal samples was determined by flow cytometry. The relative fluorescence intensity was calculated by subtracting the background counts.Back to article page