Table 2 The strains and plasmids used in this study
Strain or plasmids | Description | Source or reference |
|---|---|---|
Strains | Â | Â |
E. coli DH5? | Host for general cloning | TransGen Biotech |
E. coli TOP10 | Host for general cloning | TransGen Biotech |
E. coli S17-1 | Donor stain for conjugation between E. coli and S. spinosa | [25] |
S. spinosa ATCC 49460 | Wild strain | [26] |
S. spinosa Lu106 | S. spinosa ATCC 4946 with pLu106 | This study |
Plasmids | Â | Â |
POJ260 | E. coli Streptomcyes shuttle vector; apr oriT rep PUC lacZ | [27] |
pLu106 | pOJ260 with truncated Rex | This study |