Table 1 Strains and plasmids used in this study
Strain or plasmid | Relevant Genotype all strains are E. coli K-12 | Reference |
|---|---|---|
LJ110 | Wildtype W3110 (F−, λ−, IN (rrnD-rrnE) 1, rph-1) | |
BW25113 | lacIq rrnB T14 ∆lacZWJ WJ16 hsdR514 | [45] |
∆araBADAH33 ∆rhaBADLD78 | ||
XL1-Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac[F’proABlacIqZΔM15Tn10(Tet r )] | (Stratagene, La Jolla, USA) |
F4 | LJ 110 Δ (pheA tyrA aroF) | [38] |
F144 | LJ 110 Δlac::Ptac::aroFBL | J.Bongaerts, unpubl. [42] |
FUS4 | LJ110 Δ (pheA tyrA aroF) Δlac::Ptac::aroFBL + = F4 x P1-lysate F144 | G. A. Sprenger and N. Trachtmann, unpubl. |
BW25113 ΔtktA | BW25113 Ara+ ΔtktA | N. Trachtmann and G.A. Sprenger, [42] |
BW25113 ΔtktA tktA + | BW25113 Ara+ ΔtktA gal::Ptac ::tktA-cat | N. Trachtmann, [42] |
BW25113 glpK + | BW25113 Δmal::Ptac ::glpK-cat | This work |
BW25113 glpK G232D | BW25113 Δmal::Ptac ::glpK G232D-cat | This work |
LJ110 ΔglpK | LJ110 ΔglpK-km | This work |
LJ110 ΔglpK glpK + | LJ110 ΔglpK-km Δmal::Ptac ::glpK-cat = LJ110 ΔglpK x P1-lysate BW25113 glpK + | This work |
LJ110 ΔglpK glpK G232D+ | LJ110 ΔglpK-km Δmal::Ptac ::glpKG232D-cat = LJ110 ΔglpK x P1-lysate BW25113 glpK G232D+ | This work |
BW25113 glpX + | BW25113 Δrbs::Ptac ::glpX-cat | This work |
FUS4.2 | FUS4 Δmal::Ptac ::glpK-cat = FUS4 x P1-lysate BW25113 glpK + | This work |
FUS4.2-1 | FUS4 Δmal::Ptac ::glpKG232D-cat = FUS4 x P1-lysate BW25113 glpK fbr+ | This work |
FUS4.5 | FUS4 Δgal::Ptac ::tktA-cat = FUS4 x P1-lysate BW25113 ΔtktA tktA + | This work |
FUS4.6 | FUS4 Δrbs::Ptac ::glpX-cat = FUS4 x P1-lysate BW25113 glpX + | This work |
FUS4.6-2 | FUS4 Δrbs::Ptac ::glpX | This work |
FUS4.7 | FUS4 Δrbs::Ptac ::glpX Δgal::Ptac ::tktA-cat = FUS4.6-2 x P1-lysate BW25113 ΔtktA tktA + | This work |
pKD46 | λ Red disruption system (γ, β, exo under control of araBp), AmpR | [45] |
pCP20 | FLP + , λ ci857+, λ ρ R Repts, AmpR, CmR | [46] |
pCO1kanFRT | Ampr Kmr; Kanamycin resistance gene flanked by FRT sites | U. Degner and G.A. Sprenger, unpubl. [42] |
pCO1catFRT | Apr Cmr; chloramphenicol resistance gene flanked by FRT sites | U. Degner and G.A. Sprenger, [42] |
pJF119EH | Ptac, RBS, AmpR, lacI | [47] |
pF81 | Ptac::aroF, pheA fbr , aroB, aroL AmpR, lacI | |
pKUS3 | 1,5 kb glpK-PCR-Product (Nde I → blunt/Hind III) from E. coli LJ110 (W3110) in pJF119EH (EcoRI → blunt/HindIII) | This work |
pKUS3-1 | pKUS3 with Quikchange glpK G695A (bp) | This work, according to [48] |
pKUS10 | pKUS3 with a chloramphenicol-resistance cassette in Sph I-site | This work |
pKUS13 | 1,5 kb glpX-PCR-Product (Nde I → blunt/Hind III) from E. coli LJ110 (W3110) in pJF119EH (EcoRI → blunt/HindIII) | This work |
pKUS14 | pKUS13 with a chloramphenicol-resistance cassette in Sph I-site | This work |