Figure 4

Fed-batch cultivations of FUS4/pF81 mutants with putatively increased flux through the PPP. Fed-batch fermentations were conducted in MM with pure glycerol as carbon source. Cell growth (diamonds) and L-Phe concentrations [g l⁻¹] (squares) are shown in the diagrams a-c. The arrows indicate induction with IPTG. Data for carbon yield, space-time-yield and absolute concentration of L-Phe are listed in Table 2. All fermentations were performed two times independently, one typical growth curve is presented. (a) FUS4.6/pF81 (additional gene copy of glpX), (b) FUS4.5/pF81 (additional gene copy of tktA), (c) FUS4.7/pF81 (additional gene copy of tktA and glpX).