Figure 2

In order to compare (glucose, glycerol, crude glycerol) and pO ₂ on L-phenylalanine productivity in fed-batch cultivations. Strain FUS4/pF81 was cultivated in fed-batch mode under different conditions. (a) with glycerol at 60%, (b) or 20% O₂ saturation, (c) with glucose (60% O₂), or (d) with crude glycerol (20% O₂) as carbon source. The arrows indicate the time point when IPTG was added for induction. Data for growth rate, carbon yield, space-time-yield, and absolute concentration of L-Phe are listed in Table 2. All fermentations were conducted in MM as described in Materials and methods and were performed two times independently. The mean values are given in Table 2. Diamonds indicate OD₆₀₀ values, triangles indicate L-Phe concentration [g l⁻¹].