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Table 1 Genes used in this study

From: The important ergot alkaloid intermediate chanoclavine-I produced in the yeast Saccharomyces cerevisiae by the combined action of EasC and EasE from Aspergillus japonicus

CDS name

Source

Amino acids

dmaW_Aj1

WO2012/116935

534

dmaW_Aj2

WO2012/116935

479

dmaW_Aj3

WO2012/116935

428

dmaW_Af

XM_751048

451

dmaW_Cp

AJ011963

448

easF_Aj

WO2012/116935

340

easF_Af

XM_751050

339

easC_Aj

WO2012/116935

510

easC_Af

XM_751047

520

easC_Aj -PTS1

WO2012/116935

507

easE_Aj

WO2012/116935

622

easE_Af

XM_751049

628

easE_Cp

AJ011965

483

easE_Aj -N sig.

WO2012/116935

594

pdi1/easE_Aj

D00842/WO2012/116935

615

pdi1_Sc

D00842

522

ero1_Sc

NM_001182493

563

fad1_Sc

NM_001180104

306

  1. Gene names are followed by a two-letter code to indicate species of origin: Aj: A. japonicus; Af: A. fumigatus; Cp: C. purpurea; Sc: S. cerevisiae. All genes were synthesized with yeast codon optimization, except for pdi1_Sc, ero1_Sc, and fad1_Sc, which were prepared by PCR on yeast genomic DNA. Synthesis and PCR primer design was based on the named sources to give proteins of the indicated length.
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Microbial Cell Factories

ISSN: 1475-2859

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