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Figure 5 | Microbial Cell Factories

Figure 5

From: The important ergot alkaloid intermediate chanoclavine-I produced in the yeast Saccharomyces cerevisiae by the combined action of EasC and EasE from Aspergillus japonicus

Figure 5

Signal peptide from Pdi1 partially restores EasE function. Concentration (mg/l) of chanoclavine-I, was measured in the growth medium, of a strain expressing DmaW_Aj2, EasF_Af, and EasC_Aj after co-expression of different versions of EasE. Expression of the wt EasE_Aj (A) resulted in production of 0.75 mg/l chanoclavine-I, whereas expression of a modified version (B), comprising the N-terminal signal peptide from Pdi1, resulted in production of approximately half of this amount. Complete deletion of the N-terminal sequence (C) abolished the function of EasE_Aj, and essentially no chanoclavine-I was detected.

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Microbial Cell Factories

ISSN: 1475-2859

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