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Figure 3 | Microbial Cell Factories

Figure 3

From: The important ergot alkaloid intermediate chanoclavine-I produced in the yeast Saccharomyces cerevisiae by the combined action of EasC and EasE from Aspergillus japonicus

Figure 3

Production of chanoclavine-I enabled by EasE from A. japonicus . Yeast strains co-expressing DmaW_Aj2, EasF_Af, and EasC_Aj in combination with one of three different homologues, EasE_Aj (A), EasE_Af (B), or EasE_Cp (C), were analysed by UPLC-TOF for chanoclavine-I production. Only the strain co-expressing EasE_Aj produced chanoclavine-I, whereas no chanoclavine-I was detected in strains expressing either EasE_Af or EasE_Cp. Retention time and m/z of (A) corresponded to a chanoclavine-I reference extracted from A. japonicus mycelium (D).

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Microbial Cell Factories

ISSN: 1475-2859

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