Skip to main content
Figure 2 | Microbial Cell Factories

Figure 2

From: The important ergot alkaloid intermediate chanoclavine-I produced in the yeast Saccharomyces cerevisiae by the combined action of EasC and EasE from Aspergillus japonicus

Figure 2

Activity of different DmaW and EasF enzymes. Relative production of DMAT (black bars) and Me-DMAT (grey bars) were analysed in strains co-expressing EasF_Aj in combination with DmaW_Aj1 (A), DmaW_Aj2 (B), DmaW_Aj3 (C), DmaW_Af (D), or DmaW_Cp (E). Expression of DmaW_Aj1 and DmaW_Af resulted in only small amounts of compounds compared to the other three DmaW homologues. Similarly, DmaW_Aj2 was co-expressed with the two homologues EasF_Aj (G) or EasF_Af (H) and relative DMAT and Me-DMAT amounts were measured. The EasF_Af resulted in more than double the amount of Me-DMAT compared to EasF_Aj. The control strain (F) carried an empty plasmid with no DmaW expression. The vertical axis shows arbitrary units based on area under the HPLC peak.

Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us