Figure 3
Intracellular glycogen levels and glg expression during growth on raffinose versus trehalose. (A) Growth and glycogen accumulation profiles of L. acidophilus in semi-defined medium (SDM) [35] containing 2% of raffinose or trehalose as the sole carbon source. The glycogen levels at various growth phases (indicated by OD600) from (A) were compiled and plotted against the transcript levels of the glg operon in (B). The data represent the mean??standard deviation for two independent biological replicates. Growth phases are represented by OD600: 0.3, early log; 0.6, mid-log; 1.0, early stationary; 1.5, stationary, and 2.2, late stationary. Intracellular glycogen content was quantified by hexokinase/glucose-6-phosphate dehydrogenase-based glucose assay and expressed as mg of glucose (released from glycogen by amyloglucosidase) per g of cell wet weight (mg glucose/g cww). Figure adapted from [23].