Table 3 Characteristics of strains and plasmids used in the present study

Strains

E. coli DH5α

Φ80 lacZ ΔM15 ΔlacU169 recA1 endA1 hsdR17 supE44 thi-1 gyrA relA1

Stratagene

BY14-α17

MAT α, Industrial baker’s yeast

This study

B-MIG1

MAT α, ΔMIG1:: loxP

This study

B-TUP1

MAT α, ΔTUP1:: loxP

This study

B-SSN6

MAT α, ΔSSN6:: loxP

This study

B-MIG1-TUP1

MAT α, ΔMIG1:: loxP, ΔTUP1:: loxP

This study

B-MIG1-SSN6

MAT α, ΔMIG1:: loxP, ΔSSN6:: loxP

This study

B-TUP1-SSN6

MAT α, ΔTUP1:: loxP, ΔSSN6:: loxP

This study

B-MIG1-TUP1-SSN6

MAT α, ΔMIG1:: loxP, ΔTUP1:: loxP, ΔSSN6:: loxP

This study

Plasmids

pUG6

E. coli/S. cerevisiae shuttle vector, containing Amp ⁺ and loxP-kanMX-loxP disruption cassette

[[34]]

pUC19

Ap^r, cloning vector

Invitrogen

pSH-Zeocin

Zeo^r, Cre expression vector

[[35]]

pKAB

Ap^r, Kan^r, A_m-KanMX-B_m

[[10]]

pUC-KA _t B _t

Ap^r, Kan^r, A_t-KanMX-B_t

This study

pUC-KA _s B _s

Ap^r, Kan^r, A_s-KanMX-B_s

This study