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Figure 2 | Microbial Cell Factories

Figure 2

From: High yield expression of leptospirosis vaccine candidates LigA and LipL32 in the methylotrophic yeast Pichia pastoris

Figure 2

Expression of rLigANI and rLipL32 proteins in P. pastoris. Time courses for the expression of secreted rLigANI and rLipL32 by P. pastoris induced for up to 192 hours (8 days), (A) as determined by protein concentration (mg/mL). Western blot analysis of the intracellular (pellet) and secreted (supernatant) expression of rLigANI (B and C, respectively) and rLipL32 (D and E, respectively), using polyclonal anti-LigANI sera or anti-LipL32 Mab. Samples (cells and supernatant) were collected at the various hourly time points indicated. KM - negative control: untransformed P. pastoris KM71 H culture.

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Microbial Cell Factories

ISSN: 1475-2859

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