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Figure 1 | Microbial Cell Factories

Figure 1

From: High yield expression of leptospirosis vaccine candidates LigA and LipL32 in the methylotrophic yeast Pichia pastoris

Figure 1

Screening for P. pastoris recombinant clones expressing rLigANI and rLipL32. Colony blot analysis of transformed P. pastoris strain KM71 H with anti-6×His Mab. The t gD recombinant protein expressed in P. pastoris KM71 H was the positive control (+) and untransformed P. pastoris KM71 H was the negative control (-). Spots 1-7 are representative rLigANI colonies and 8-12 are representative rLipL32 colonies. Arrows indicate the colonies that were selected for large-scale expression studies.

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Microbial Cell Factories

ISSN: 1475-2859

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