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Table 3 Primers used in this study

From: Two distinct regions in the model protein Peb1 are critical for its heterologous transport out of Escherichia coli

Primer

Application

Sequence 5'-3'

Peb1RXbaI

PCR

GGCTCTAGATTATAAACCCCATTTTTTCGC

5'UTRfwBglII

PCR

GCCAGATCTGCGGGAATAAGGGGCAGAG

K1012R

PCR

GGTCTAGATTAACCCTGCAGCAGAGACA

FliC183XbaIR

PCR

GGTCTAGATTAAGTGGTAACTGTATCGTTAT

K1008OF

PCR

GGCGATCGGCGGGAATAAGGGGCAGAG

ORpLA15

PCR

GCCGCATGCCGATAAACAGCCCTGCGTTA

pBRAhdIF

Sequencing

GCCTTCGTTCATCCATAGTTGCCT

pLA21R

Sequencing

GCCCAGTTAATCAGGTTACAACGATT

NotI mini

Sequencing

TGCGGCCGCA

028R

Sequencing

CAATTCAACTTGTAGGCCTGATA

peb1/pLA25FIN

Construction of: pLA32

GATCACTCAAAATAATATCAACAAGGCAGAAGGTAAACTTGAGTC

peb1/pLA25RIN

pLA32

GACTCAAGTTTACCTTCTGCCTTGTTGATATTATTTTGAGTGATC

HotNIF

pTAR1P

TAGCTGTTGCCGCATTGCTAGCTGCAAGTATATTGGGTG

HotNIR

pTAR1P

GCAATGCGGCAACAGCTACTGCGAAACCTTTAATTTCAC

HotCIF

pTAR2P

CGTTTGCTGTAGCCGCAGCAATATTGTTAGGTTATGTGG

HotCIR

pTAR2P

ATATTGCTGCGGCTACAGCAAACGCATCAACTCTTTTAG

PebGlyF

pTAR15G

GGCGGTGGTGGGGGCGTTGCCAAATTGCTAGCTAAAAG

PebGlyR

pTAR15G

GCCCCCACCACCGCCATCTACTTCGAAACCTTTAATTTC

PebAla1F

pTAR1A

GCAGCAGCGGCGGCCGCAGCGCTAGCTAAAAGTATATTGGGTG

PebAla1R

pTAR1A

CGCTGCGGCCGCCGCTGCTGCGAAACCTTTAATTTCACCTGTTG

PebGly1F

pTAR1G

GGCGGCGGTGGTGGCGGTGGCCTAGCTAAAAGTATATTGGGTG

PebGly1R

pTAR1G

GCCACCGCCACCACCGCCGCCGAAACCTTTAATTTCACCTGTTG

PebAla3F

pTAR2A

GCAGCCGCAGCAGCAGCGGCAGCGGCGGTGGATGATAAAAGTGAAATTTTG

PebAla3R

pTAR2A

CGCCGCTGCCGCTGCTGCTGCGGCTGCAGAAAACGCATCAACTCTTTTAG

PebGly3F

pTAR2G

GGCGGCGGTGGTGGCGGGGGCGGTGGTGTGGATGATAAAAGTGAAATTTTG

PebGly3R

pTAR2G

ACCACCGCCCCCGCCACCACCGCCGCCAGAAAACGCATCAACTCTTTTAG