Skip to main content

Table 1 Bacterial strains and plasmids used in this study

From: Two distinct regions in the model protein Peb1 are critical for its heterologous transport out of Escherichia coli

Strain or plasmid Relevant characteristics Source or reference
Strains   
   E. coli AAEC072A MG1655 ΔfimA-H [44]
   E. coli MKS12 MG1655 ΔfimA-H ΔfliCD [21]
   E. coli MKS12P lac peb1 MKS12 carrying peb1 under P lac at the ΔfliC site This study
   E. coli MKS111 MG1655 ΔfimA-H ΔfliCD ΔflgM This study
   E. coli MKS111ΔHBB MG1655 ΔfimA-H ΔfliCD ΔflgM ΔfliFGH ΔflgDEFGHIJ This study
   E. coli DH10B endA- RecA- Dam/Dcm+ [66]
Plasmids   
   pMCS3'UTR Multiple cloning site preceding the 3'UTR sequence of fliC in Pvu I-EcoR I of pBR322 [21]
   pPeb15'3' 173 bp fliC promoter sequence and 702bp fragment encoding mature Peb1 in Pvu I-Sca I site of pMCS3'UTR [21]
   pLA25 PfliC and fragment encoding mature Peb1, named fliC 5'UTR-peb1, in BglI I-Xba I site of pMCS3'UTR This study
   pLA26 3'UTR removed by digestion at Sca I-EcoR I sites of pLA25 This study
   pLA31 Fragment containing PfliC and truncated fliC (encoding N-terminal 183 aa) in BglI I-Xba I site of pMCS3'UTR This study
   pLA32 Fragment containing PfliC, truncated fliC (encoding N-terminal 20 aa) and peb1 (without ss) in BglI I-Xba I site of pLA25 This study
   pTAR15G Insertion of five glycines at residue D42 of Peb1. pLA25 background. This study
   pTAR1P Substitution of four separate polar residues in Peb1 TAR1 region with alanine. pLA25 background. This study
   pTAR2P Substitution of four separate polar residues in Peb1 TAR2 region with alanine pLA25 background. This study
   pTAR1A Substitution of seven residues in Peb1 TAR1 region with alanine. pLA25 background. This study
   pTAR1G Substitution of seven residues in Peb1 TAR1 region with glycine. pLA25 background. This study
   pTAR2A Substitution of eight residues in Peb1 TAR2 region with alanine. pLA25 background. This study
   pTAR2G Substitution of eight residues in Peb1 TAR2 region with glycine. pLA25 background. This study