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Figure 5 | Microbial Cell Factories

Figure 5

From: Two distinct regions in the model protein Peb1 are critical for its heterologous transport out of Escherichia coli

Figure 5

Subcellular localization of Peb1 mutants. SDS-PAGE (top panel) and Western blot (middle panel) analysis with anti-Peb1 antibodies of whole-cell samples and periplasmic extracts of MKS12 carrying the plasmids indicated. Cell lysis and cytoplasmic leakage was controlled by immunoblotting with anti-RNAP antibodies (bottom panel). Plasmids p9D5 and p8C3 carry insertions with no or minor effect on Peb1 expression and secretion efficiency, pTAR1A and pTAR2A carry long Ala substitutions in the corresponding TAR regions. Note the presence of Peb1 and its mutant derivatives in the periplasm of MKS12. The letter C indicates whole-cell samples, S cell-free culture supernatants, and PP periplasmic extracts. All the samples correspond to 100 μl of culture and molecular weight markers in kDa are indicated on the left.

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