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Figure 7 | Microbial Cell Factories

Figure 7

From: Differential proteomic analysis highlights metabolic strategies associated with balhimycin production in Amycolatopsis balhimycina chemostat cultivations

Figure 7

Gel band shift assays. EMSA was carried out using DNA fragments upstream A. balhimycina ppk (Pppk Amy) and pstS (PpstS Amy) and a crude extract from A. balhimycina LP culture. The use of a molar excess (200 fold) of unlabeled probe as well as of a DNA fragment upstream S. coelicolor pstS (PpstS Sco), containing a PHO box, reduced the band shift signals for both probes. No reduction of band shift signals is visible using a molar excess (200 fold) of an unspecific competitor (Nonomuraea Pdbv14 [14]).

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Microbial Cell Factories

ISSN: 1475-2859

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