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Figure 4 | Microbial Cell Factories

Figure 4

From: Fed-batch fermentation of GM-CSF-producing glycoengineered Pichia pastoris under controlled specific growth rate

Figure 4

DSA-FACE analysis of the total N-glycan pool from Man5mGM-CSF strain. Panel 1 shows the results for a malto-dextrose reference. Panels 2 through 5 show the results for N-glycans, as follows: panel 2: GS115mGM-CSF (the main peak is Man9GlcNAc2 [M9]); panel 3, Man5mGM-CSF (the predominant peak is Man5GlcNAc2; a minor fraction of higher molecular weight structures is marked with an asterisk); panel 4, same as panel 3 but the N-glycans were treated with α-1,2-mannosidase; panel 5, reference N-glycans from bovine RNase B (Man5-9GlcNAc2 [M5-M9]).

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