Skip to main content

Table 2 Oligonucleotides used in this study

From: Dissection of an old protein reveals a novel application: domain D of Staphylococcus aureus Protein A (sSpAD) as a secretion - tag

Primers

Sequences (5'- 3')

pET 30 sSpAD NdeI F

GCACGACATATGGCAGACGCACAACAGAATAAG

pET 30 sSpAD NdeI R

TAGCAGCATATGTTTTGGTGCCTGGAGTTC

pLacUV5 sSpAD NheI R

GCAAGCTAGCTTTTGGTGCCTGAGATTCGTTC

SOD NheI F

TAAAGCTAGCGCGGCAACAAAGGCCGTGTG

SOD SalI R

AGTTGTCGAC TTGGGCGATCCCAATTACACC

sGFP F NdeI

GGATCCACTCATATGAGCAAAGGCGAAG

sGFP R

CGAGGTCGACTTATTATTTATACAGTTCATC

prom Tac SphI F

5'P-CGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTT

prom Tac XbaI R

5'P- CTAGAAATTGTTATCCGCTCACAATTCCACACATTATACGAGCCGATGATTAATTGTCAACAGCTCGCATG

prom LacUV5 SphI F

5'P-CCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTT

prom LacUV5 XbaI R

5'P-CTAGAAATTGTTATCCGCTCACAATTCCACACATTATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGCATG

T5 Prom SphI F

GGCGGCATGCGAAATCATAAAAAATTTAT

T5 Prom XbaI R

TTTCTAGATGTGTGAAATTGTTATCCGCT

  1. a The oligonucleotides contain the letters: F - forward or R - reverse
  2. 5'P represents a 5'Phosphate group modification