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Table 1 Secretion capacity of the fusion proteins

From: Dissection of an old protein reveals a novel application: domain D of Staphylococcus aureus Protein A (sSpAD) as a secretion - tag

Vectors

Fusion Protein

Secretion Capacity

pLacUV5a

sSpAD-NproEDDIE-pep6His

11.3 mg/L

pLacUV5

sSpAD-Gfpmut3.1

1.3 mg/L

pLacUV5

sSpAD-SOD

16.4 mg/L

  1. a pET30a plasmids with the lacUV5 promoter are named pLacUV5