Figure 4From: Dissection of an old protein reveals a novel application: domain D of Staphylococcus aureus Protein A (sSpAD) as a secretion - tagIgG column purification of an sSpAD-fusion protein. sSpAD-SOD was purified out of the periplasmic fraction of a shaking flask cultivation in BL21(DE3). P: periplasmic fraction, Fl: flow through, W: washing step, E1-E4: eluted fractions.Back to article page